Evaluation of the Effects of Biomaterial Scaffold for Healing Cutaneous Chronic Wounds in Dog Model

Abstract

Therapeutic assessment of the biomaterial scaffoldutilized as a surgical adjunct to keep up the inflammatory process and to potentiate tissue healing, make the subject of ongoing research in regenerative medicine. This study was intended to assess the healing activity of effect of lyophilized bovine urinary bladder sub mucosa (BUBM) by topical application on the skin experimentally in chronic full - thickness cutaneous wounds in a dog model. For this reason, thisinvestigation was conducted on twenty adult and clinically healthy males'dogs, full-thickness square skin wounds (4×4 cm) and 10 cm apart were made on the back of each animal. After surgical creation these injuries were confronted every daily surgical scratching to interrupt healing process continuation to prolong inflammatory reaction to form chronic wound. The latter surgical procedure was continued for eight weeks to make certain of their change to chronic wounds. The animals were arbitrarily divided into two equal groupsof ten dogs for each.In control group, the cranial injuries were left without treatment.While in scaffold group, and the caudal injuries were treated by implantation 0.08 mg (BUBM) powder. The clinical assessment of treated wounds demonstrated that the injury healing process contraction%, Re-epithilization % and total wound healing % were significantlyP<0.05 than that of control wounds at forty five days of the investigation. The histopathological studying on seventh, fourteen, 28 and 45 days post-treatment demonstrated that treated wounds have reduces inflammation during 3 first days post-implantation and promotes epithelialization in 3 weeks of healing withincreased vasculature than those in untreated wounds.This study concluded the continuous mechanical irritation of wounds site may lead to form chronicity state for the effected wounds and topical administration lyophilized bovine urinary bladder sub mucosa (BUBM) enables us toincrement and improve the therapeutic approach tothe chronic cutaneous wound.