Cell-free protein synthesis: Search for the happy middle

Abstract

Cell-free protein synthesis (CFPS) is a versatile technique gaining popularity because it allows researchers access to on-demand production of proteins. This Commentary by Zachary Russ and John Dueber discusses the latest research article by Rui Gan and Michael Jewett, which reports a convenient and cost-effective methodology for the preparation of Saccharomyces cerevisiae-based CFPS reactions. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.