Preparation and Characterization of Bacterial Cellulose from Culturation of Acetobacter Xylinum in Coconut Water Media

Abstract

This study aims to analyze bacterial cellulose obtained from culturing Acetobacterxylinum in coconut water media. Coconut water media is obtained from coconut water waste in existing traditional markets. The resulting nata de coco purified with a 1% (w / v) solution of sodium hydroxide (NaOH) and 1% (v / v) acetic acid (CH3COOH). The preparation process, bacterial cellulose in the form of thin sheets, is dried under the sun or in an oven at 60 ° C; after drying, it is mashed in a blender until a solid powder is obtained. The resulting bacterial cellulose then characterized using Fourier Transform Infrared (FTIR), Thermo Gravimetric Analysis (TGA), and Scanning Electron Microscopy (SEM). The results of FTIR analysis showed that the cellulose obtained derived from microorganism activity; TGA analysis showed that the weight loss was 9.2%; this indicates the hydrophilic nature of cellulose. SEM analysis shows that bacterial cellulose's surface morphology looks very dense and dense; this shows the characteristic properties of bacterial cellulose.