Isolating Venomous Constituents of Paralepistopsis acromelalga and Simultaneous Analysis of Its Compounds

Abstract

An isolation method for Acromelic acids A, B and Clitidine, which are venomous constituents of Paralepistopsis acromelalga was developed. Highly purified products were obtained from the mushroom extract using silica gel, ODS, ion-exchange column chromatography and preparative TLC. Using those results, we optimized the LC-MS/MS conditions. Finally we developed a method for simultaneous analysis. In recovery tests, the average recovery was 80.8–112.4％, repeatability was 1.4–3.8RSD％. The limits of quantification of the respective compounds were estimated as 0.25 μ g/g. Based on the results, this method can reveal causes of food poisoning by Paralepistopsis acromelalga.