Background: Mounting evidence in the cancer literature suggests that microRNAs (miRNAs) influence the progression of human cancer cells by targeting protein-coding genes. How insulin-like growth factor 1(IGF1) and miR-186-3p contribute to the development of cervical cancer (CC) remains unclear. This study examined the regulatory roles of miR-186-3p and IGF1 in CC development. Methods: Gene expression levels were determined by qRT-PCR. Proliferation, migration, and apoptosis of CC and normal cells were determined by MTT, Transwell, and caspase-3 activity assays, respectively. Dual-luciferase reporter activity and RNA pull-down assays were performed to identify the target gene of miR-186-3p. Results: IGF1 was the target of miR-186-3p. The expression of miR-186-3p inhibited cell proliferation and migration abilities of CC cell lines, but induced the apoptosis rate of CC cells. IGF1 could restore the inhibitory effects of miR-186-3p on the proliferation, migration, and apoptosis abilities of CC cells. Experimental results revealed that miR-186-3p could inhibit IGF1 expression, thereby reducing the viability of CC cells. Conclusions: The data suggest that targeting of IGF1 by miR-186-3p could be crucial in regulating the progression of CC.
CITATION STYLE
Lu, X., Song, X., Hao, X., Liu, X., Zhang, X., Yuan, N., … Zhang, Z. (2021). MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1. World Journal of Surgical Oncology, 19(1). https://doi.org/10.1186/s12957-021-02317-z
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