Total abdominal 18F-FDG uptake reflects intestinal adenoma burden in Apc mutant mice

Abstract

Apc mutant (ApcMin) mice develop multiple adenomas in their intestines and are widely used to study colorectal carcinogenesis and chemopreventive approaches. Molecular imaging of intestinal adenomas could potentially provide noninvasive longitudinal evaluation of these lesions in living mice. Therefore, the aim of this study was to investigate the role of 18F-FDG PET in the ApcMin mouse model. Methods: Apc Min mice (n = 8) fed a purified diet were imaged serially after injection of 18F-FDG at age 9 and 12 wk using a small-animal PET scanner. Abdominal uptake of the tracer was quantified. After dissection, intestines were imaged separately, and intestinal tracer uptake was quantified. Tracer distribution was compared with results from microscopic examination regarding adenoma number and size. Thereafter, findings were validated serially in 20 ApcMin mice aged 6, 8, 10, and 12 wk that received standard chow to increase adenoma numbers. In vivo abdominal 18F-FDG uptake was correlated with microscopy results. Results: Microscopic examination showed that the mice developed 25-35 intestinal adenomas at age 12 wk. Ex vivo 18F-FDG PET of the dissected intestines visualized all large adenomas and most small adenomas. Ex vivo total intestinal 18F-FDG uptake correlated with in vivo total abdominal uptake and with the number of large adenomas at age 9 and 12 wk. At 12 wk, there was a clear correlation between in vivo abdominal tracer uptake and the number of large adenomas but not the total number of lesions. Conclusion: Intestinal adenomas in ApcMin mice are metabolically active lesions that take up 18F-FDG. Abdominal 18F-FDG uptake at age 12 wk serves as a readout modality for large intestinal adenomas. Copyright © 2011 by the Society of Nuclear Medicine, Inc.