HTLV-2: uma infecção antiga entre os povos indígenas da Amazônia brasileira

Abstract

Human T-cell lymphotropic viruses 1 and 2 (HTLV-1 and HTLV-2) were the first human retroviruses to be described causing diseases in humans; they were isolated in lymphocyte cultures and share numerous similarities in biological and molecular properties 1,2. More recently, two new types, the human T-cell lymphotropic virus 3 (HTLV-3) and the human T-cell lymphotropic virus 4 (HTLV-4), were described in populations of the Republic of Cameroon, the former being associated with Primate T-lymphotropic virus 3 (PTLV-3) and the latter showing no phylogenetic relationship to the HTLV and PTLV types known so far 3,4. HTLV-3 and HTLV-4 are restricted to few cases among hunters, animal meat handlers and are still waiting for further evidences of human dissemination elsewhere. Molecular studies revealed the existence of a genetic variant of HTLV-2 among the Kayapó group, called HTLV-2c 5 , being distinct from the subtypes 2a and 2b described, until then, in other human populations 6. The occurrence of subtype 2c has been confirmed in several villages of the Kayapó group, as well as among the Arara do Laranjal, Zo'é and Tiriyó tribes 5,7,8,9. For many years, the endemic presence of HTLV-2 among indigenous communities in the Americas supported the theory of origin for this virus in the New World 10. This hypothesis became attractive when Chen et al. 11 isolated the Primate T-lymphotropic virus 2 (PTLV-2) in two species of New World monkeys, suggesting the transmission due to interspecific barrier crossing. However, new studies, conducted in primates from the Americas, were not successful in demonstrating evidences of PTLV-2 infection, ruling out the American origin hypothesis for HTLV-2. The presence of HTLV-2b infection in Central African pygmy peoples 12 supports the hypothesis that the human infection with HTLV-2 is old and that it began on the African continent, followed by its spread to Europe and Asia through human migratory movements initiated some 100,000 years ago 13. The finding of the new subtype HTLV-2d with high degree of genetic divergence in pygmies from the Democratic Republic of Congo has reinforced this hypothesis 14. Likewise, the phylogenetic proximity between African and Amerindian HTLV-2a strains suggests that the evolution of HTLV-2a and HTLV-2b preceded the human migratory movement from Africa toward Europe and Asia. This is supported by the phylogenetic analysis which reveal that viral isolates in Africa are represented by all three subtypes (2a, 2b and 2d), showing the large heterogeneity of the virus in the continent. The evidence of HTLV-2a in an isolated human community in Mongolia 15 suggested that the virus was disseminated following the human migratory movement towards Asia, a route that gave rise to Amerindian populations around 10,000 to 40,000 years. This hypothesis has also been strongly accepted to explain the endemic presence of HTLV-2b in several indigenous populations in the Americas 13. The migratory waves towards the Americas have been reviewed and, according to archaeological, anthropological and genetic findings, the migrations of Amerindian ancestors occurred according to two or more independent routes 16. Equally, the migratory movements towards South America had two possible routes: one towards the Amazon and the other down the Pacific coast, parallel to the Andes Cordillera 16,17. This differential migratory flow of Amerindian ancestors to South America may have introduced HTLV-2c in the Amazon Region, characterizing a typical founder effect. On the other hand, as shown by the phylogenetic analysis, viral samples identified among Amerindian and urban groups (HTLV-2c) are distinct from those isolated from HTLV-2a found in North America 5,9. Among other possible explanations for the origin of HTLV-2c in the Amazon, there is the possibility of an autochthonous origin, from a proto-HTLV-2a infecting an Ameridian ancestral who entered Brazil through the Amazon, and the new molecular subtype disseminated among the Indian peoples of Brazil as a result of the process of population expansion. Many of the Indian populations of South America have been isolated, or establishing few interactions with other human groups (indigenous or not). This suggests that HTLV-2c infection is ancient, and probably existed in the present form or evolved during its migration together with their human ancestors to the New Continent. The genetic similarity of more than 99% among HTLV-2c strains, isolated from urban and indigenous areas, gives supports to the suggestions that the virus was actively transmitted to urban areas, increasing its geographic endemicity within the Amazon Region 9 .