Hydrogenophaga temperata sp nov., a betaproteobacterium isolated from compost in Korea

Abstract

A Gram-negative, rod-shaped, non-spore-forming and motile bacterial strain TR7-01T was isolated from a compost soil in South Korea and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TR7-01T belonged to the genus Hydrogenophaga within the class Betaproteobacteria. Strain TR7-01T exhibited 16S rRNA gene sequence similarity values of 95.0 98.3% to members of the genus Hydrogenophaga: Hydrogenophaga bisanensis DSM12412T (98.3%), Hydrogenophaga flava DSM 619T (97.1%), Hydrogenophaga pseudoflava ATCC 33668T (96.8%), Hydrogenophaga intermedia S1T (96.4%), Hydrogenophaga atypica BSB 41.8T (95.8%), Hydrogenophaga defluvii BSB 9.5T (95.7%), Hydrogenophaga palleronii CCUG 20334T (95.6%), Hydrogenophaga caeni EMB71T (95.4%) and Hydrogenophaga taeniospiralis ATCC 49743T (95.0%). Chemotaxonomic data revealed that strain TR7-01T possesses ubiquinone Q-8, the G+C content was 69.9 mol%, and the predominant fatty acids were 16:ω7c/15:0 iso 2OH, 18:1ω7c/ω 9t/ω 12t and C16:0, all of which corroborated our assignment of the strain to the genus Hydrogenophaga. The results of DNA DNA hybridization and physiological and biochemical tests clearly demonstrated that strain TR7-01T represents a distinct species. Based on these data, TR7-01T (= KCTC 12203T = DSM 18117T) should be classifi ed as a novel Hydrogenophaga species, for which the name Hydrogenophaga temperata sp. nov. has been proposed.