C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells

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Abstract

We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells. © 2014 Zevnik et al.

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Zevnik, B., Uyttersprot, N. C., Perez, A. V., Bothe, G. W. M., Kern, H., & Kauselmann, G. (2014). C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS ONE, 9(3). https://doi.org/10.1371/journal.pone.0090570

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