Effect of liquid biological fertilizer on dwarf coconut embryo culture

Abstract

Organic or biological fertilizer liquid is generally used to improve crop quality in plants grown in-vivo. Liquid organic and inorganic fertilizers have been used as in vitro culture media for orchids and patchouli; it is suspected that liquid biological fertilizers can also be used as in vitro growth medium. The research was conducted to determine the effect of liquid biological fertilizer (LBF) on the growth medium and development of dwarf coconut embryo culture. The study was conducted in laboratory tissue culture Palma Crops Research Institute, December 2020 - May 2021. The study used live combinations of culture mediums, and each used ten explants of dwarf coconut embryos. The results showed that on Eeuwens Y3 100% + 100% sugar medium, all experienced browning, three green shoots (live), and seven brown shoots (dead), the average shoot length was 2.9 cm. Medium LBF 100% without sugar does not undergo browning, and the embryo does not develop. LBF 50% + Eeuwens Y3 50% + 50 % sugar best medium: a minor browning, the highest number of green shoots (8 shoots), average shoot length was 4.1 cm.