Antimicrobial activity assay of Plantaricin F produced by L. lactis pNZ8148-plnAF against Candida albicans

Abstract

Plantaricin is a bacteriocin produced by Lactobacillus plantarum. It has potential as probiotic, antimicrobial, easily degraded by proteolytic enzyme and Generally Recognized as Safe (GRAS). The objective of this study was to produce plantaricin F expressed from Lactococcus lactis pNZ8148-plnAF, purified using gel-filtration chromatography and investigated its antimicrobial activity using disc diffusion method against Candida albicans using nystatin as a positive control. Crude plantaricin F yield was 1.23 grams. Gel-filtration chromatography using Sephadex G-50 of plantaricin F yielding in 25 fractions. 5 fractions (fraction no 7, 9, 17, 18, 19) from 25 fractions have antimicrobial activities based on the clear zone on disc diffusion method.