Optimalization and regeneration of in vitro seedling of Shallot variety Lembah Palu in providing good quality seedling

Abstract

This research aimed to determine suitable bulb size and proper concentration of benzylamino purine (BAP) to produce vigor plantlets. The research was arranged as factorial completely randomized design with two factors. The first factor was bulb diameter with three levels (0.8, 1.0 and 1.2 ± 0.05 cm). The second factor wasBAP concentration with three levels (2, 4 and 6 mg/L). All treatments were replicated 4 times, thus there were 36 experimental units. Each unit consisted of 3 explants, so there were 108 samples. The results showed that largest bulb of 1.2 cm ± 0.05 in diameter and addition of BAP 4 mg/L to Murashige Skoog basal medium were excellent for producing vigour planlets. There were interactions between the two treatments on number of leaves and root paremeters.