Abstract
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
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Spositto, F. L. E., Campanerut, P. A. Z., Ghiraldi, L. D., Leite, C. Q. F., Hirata, M. H., Hirata, R. D. C., … Fressatti Cardoso, R. (2014). Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex. Brazilian Journal of Microbiology, 45(3), 841–843. https://doi.org/10.1590/S1517-83822014000300012
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