Metabolomics Analysis of Lettuce (Lactuca sativa L.) Affected by Low Potassium Supply

Abstract

Lettuce is a commercially significant leafy vegetable worldwide. Potassium (K) is an essential macronutrient for lettuce growth and development and significantly impacts its metabolites. Biomarkers that are indicative of variations in the K status of lettuce before the occurrence of biophysical changes (e.g., leaf or canopy morphological, textural and color features), can be adopted to determine the early K status of lettuce. To understand the effect of low K on diverse metabolites, we examined the metabolic response of lettuce in a closed cultivation room under controlled conditions. The evaluation was based on large-scale untargeted metabolomics assay of the K group using liquid chromatography-mass spectrometry. Data were analyzed with a fold-change (FC) analysis, t-test, and orthogonal partial least square-discriminant analysis. Fifty-two metabolites were classified into two groups by the FC, p, and the variable importance in projection (VIP). Low K led to an increment in 40 metabolites (FC > 2, p < 0.05, VIP > 1) and a decrease in 12 metabolites (FC < 0.5, p < 0.05, VIP > 1). Ten pathways were significantly enriched with metabolic biomarkers. In light of the complex interactive relationships among N, P, and K, the top five biomarkers were screened further by VIP > 4.00. Adenosine, FA 18:1+3O, uridine, cis-aconitate, and D(-)-gulono-gamma-lactone showed an increase in low-K stress samples, and may be considered potential metabolic biomarkers. This study validates the impact of low K on lettuce metabolism, and identifies biomarkers that can be used to monitor the K status in lettuce.