Trichoderma spp no biocontrole de Cylindrocladium candelabrum em Mudas de Eucalyptus saligna

Abstract

This study tested the effects of commercial biocontrol products on in vitro and in vivo trials against the fungal pathogen Cylindrocladium candelabrum Viegas, leaf blight agent on Eucalyptus saligna. The in vitro tests (direct confrontation and inoculation on detached leaves of E. saligna) were composed by the following treatments: T1 - only C. candelabrum, T2 - 06006S isolate x C. candelabrum, T3 - 53RR isolate x C. candelabrum, T4 - 5D isolate x C. candelabrum; T5 -Agrotrich® x C. candelabrum and T6 - Trichodel® x C. candelabrum. All isolates were efficient in controlling the growth of the pathogen during the direct confrontation test; similarly, Trichoderma spp. 53RR and 06006S, as well as the commercial product Trichodel ®, controlled the symptoms on the detached leaves. In order to complement the in vitro tests, the commercial products Agrotrich® and Trichodel® were tested on E. saligna seedlings cultivated in greenhouse. The following treatments were applied: T1 - control - plants without inoculation; T2 - plants inoculated with C. candelabrum; T3 - plants inoculated with C. candelabrum x Agrotrich®; T4 - plants inoculated with C. candelabrum x Trichodel®; T5 - plants inoculated with Agrotrich® and T6 - plants inoculated with Trichodel®. Trichodel® reduced C. candelabrum damage on E. saligna seedlings.