A new monoepoxylignan from Valeriana officinalis

Abstract

Objective: To study the chemical constituents from Valeriana officinalis and their cytotoxic activities. Methods: The compounds were separated by silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-preparative HPLC. The structures were identified by their physicochemical properties, MS, 1D and 2D NMR spectra and ECD data analysis. The cytotoxic activities were tested by MTT assay. Results: Fifteen compounds were isolated from V. officinalis, which were identified as: valeofficinlignan A (1), (7R, 8S, 8'R)-tetrahydro-7-(4-hydroxy-3-methoxyphenyl)-8'-[(4'-hydroxy-3'-methoxyphenyl)methyl]-8- (hydroxylmethyl)-8, 8'-furandiol (2), schisanpropinin (3), (+)-isolariciresinol (4), ecdysanol B (5), (-)-3, 4-dihydroxy-2- (4-hydroxy-3-methoxyphenyl)-4-(4-hydroxy-3-methoxybenzyl)-3-tetrahydrofuranmethanol (6), jatamanin A (7), jatamanin K (8), jatamanin R (9), longiflorone (10), ferulic acid (11), caffeic acid (12), p-coumaric acid (13), methylcaffeate (14), and methyl p-hydroxycinnamate (15). Compound 10 showed cytotoxic activity against HCT116 cell line with IC50 value of (70.9 ± 6.4) μmol/L. Conclusion: Compound 1 is a new monoepoxylignan, namely valeofficinlignan A, and compounds 2, 3, 5-10 are isolated from V. officinalis for the first time, compound 10 exhibits cytotoxic activities.