Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

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Abstract

Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

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Berridge, B. R., Fuller, J. D., De Azavedo, J., Low, D. E., Bercovier, H., & Frelier, P. F. (1998). Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer. Journal of Clinical Microbiology, 36(9), 2778–2781. https://doi.org/10.1128/jcm.36.9.2778-2781.1998

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