Evaluation of spray and oral delivery of Newcastle disease I2 vaccine in chicken reared by smallholder farmers in central Ethiopia

Abstract

Background: Newcastle disease (ND) is a highly infectious disease causing considerable economic losses to poultry farmers worldwide. Conventional vaccine delivery methods are not suitable for smallholder and rural poultry producers, and thus appropriate vaccination methods need to be sought. This study was carried out with the main objective of evaluating the efficacy of ND I2 vaccine delivered via drinking water and spray under smallholder farmers' condition in Minjar-Shenkora district, central Ethiopia. Twenty households were randomly assigned to intervention and control groups. Chickens owned by the selected households were randomly assigned to one of the three intervention groups. Blood samples were collected regularly for antibody assay from individual chicken vaccinated with ND I2 vaccine using different routes. Results: At baseline, there was no difference in antibody titer among the experimental groups. After the first and booster vaccinations, the three vaccinated groups had significantly higher antibody titer (P < 0.001) than the unvaccinated control group. Interestingly, there was no statistically significant difference in antibody titer among the vaccinated groups. Out of the 40 chicken in the unvaccinated control only 14 had antibody titter≥ log23. Similarly 19/37 of chicken in the drinking water group, 19/37 of chicken in the eye drop group and 20/40 chicken in the spray group had antibody titer ≥ log23. Two weeks after the first vaccination the proportion of chicken with antibody titer ≥ log23 rose to 23/37, 30/37 and 29/40 in the group vaccinated via drinking water, eye drop and spray, respectively. The proportion remained low in unvaccinated group. Hundred percent of the vaccinated chicken survived after infection with the virulent ND virus (Alemaya strain); whereas only 40% survived from the unvaccinated control group. Conclusion: The results of this study showed that ND I2 vaccine administered via drinking water and spray under smallholder farmers' situation provoked protective antibody level similar to the eye drop method. The use of ND I2 vaccine could contribute to food security if used by rural poultry farmers properly.