Abstract
Abstrak Daun waru (Hibiscus tiliaceus) adalah salah satu jenis tanaman yang mengandung flavonoid dan senyawa fenolik alami serta bermanfaat sebagai antioksidan. Flavonoid dan fenolik dapat diekstraksi dengan optimal jika menggunakan pelarut ekstraksi yang sesuai. Penelitian ini bertujuan untuk mengetahui kadar flavonoid total, fenolik, dan antioksidan ekstrak daun waru menggunakan beberapa pelarut. Pengujian kadar flavonoid total dengan metode kolorimetri AlCl3, kadar fenolik total dengan metode Folin-Ciocalteu, dan kadar antioksidan dengan metode DPPH. Kadar flavonoid total ekstrak dengan pelarut metanol, etanol, dan air secara berurutan adalah 121,630 ± 2,014 µgQE/g; 133,190 ± 1,434 µgQE/g; 78,320 ± 1,799 µgQE/g. Kadar fenolik total ekstrak dengan pelarut metanol, etanol, dan air secara berurutan adalah 9.151,480 ± 58,969 µgGAE/g; 7.978,610 ± 84,845 µgGAE/g; 4.789,690 ± 14,576 µgGAE/g. Ekstrak dalam pelarut metanol memiliki aktivitas antioksidan sangat lemah (IC50 = 710,680 ± 3,747 µg/mL). Ekstrak dalam pelarut etanol memiliki aktivitas antioksidan sangat lemah (IC50 = 989,150 ± 0,798 µg/mL). Ekstrak dalam pelarut air memiliki aktivitas antioksidan sangat lemah (IC50 = 1.643,480 ± 1,828 µg/mL). Ekstrak etanol memiliki kadar flavonoid total tertinggi. Ekstrak metanol memiliki kadar fenolik total tertinggi dan aktivitas antioksidan sangat lemah.. Abstract Waru leaf (Hibiscus tiliaceus) is plant that contains natural flavonoids and phenolic compounds and is useful as an antioxidant. Flavonoids and phenolics can be extracted optimally if using a suitable extraction solvents. This study aims to determine the total flavonoid, phenolic, and antioxidant levels of waru leaf extract using several extraction solvents. The total flavonoid content was tested using the AlCl3 colorimetric method, total phenolic content using the Folin-Ciocalteu method, and antioxidant levels using the DPPH method. The total flavonoid content of the extract with methanol, ethanol, and water solvents respectively 121,630 ± 2,014 gQE/g; 133,190 ± 1,434 gQE/g; 78.320 ± 1.799 gQE/g. The total phenolic content of the extract with methanol, ethanol, and water solvents were 9,151,480 ± 58,969 gGAE/g; 7,978,610 ± 84,845 gGAE/g; 4,789,690 ± 14,576 gGAE/g. Extract with methanol solvent has very weak antioxidant activity (IC50 = 710,680 ± 3,747 g/mL). Extract with ethanol solvent has very weak antioxidant activity (IC50 = 989.150 ± 0.798 g/mL). Extract with water solvent has very weak antioxidant activity (IC50 = 1,643,480 ± 1,828 g/mL). Ethanol extract had the highest total flavonoid content. Methanol extract had the highest total phenolic content and very weak antioxidant activity.
Cite
CITATION STYLE
Oktavia Rahayu, L., Kartika Putri, O., & Daniar Manggarani, R. (2022). Kadar Flavonoid dan Fenolik Ekstrak Daun Waru (Hibiscus tiliaceus) Serta Aktivitasnya Sebagai Antioksidan. JC-T (Journal Cis-Trans): Jurnal Kimia Dan Terapannya, 6(1). https://doi.org/10.17977/um0260v6i12022p017
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