Polyhydroxyalkanoates production by Zobellella species isolated from fish industrial effluents and its primary characterization

Abstract

Aim : The present study aimed to produce and characterize polyhydroxyalkanoates by exploring high yielding bacterial isolatesfromfishindustrialeffluents. Methodology: Industrial effluents samples were collected and processed forphysico-chemical parameter and bacteriological analysis. The polyhydroxyalkanoates production efficacies of bacterial isolates were evaluated using submerged fermentation process and its primary characterization was carried out using fourier transform infra-red spectroscopy. Results: Out of thirty three, five bacterial isolates showed accumulation of polyhydroxyalkanoates granule under Sudan black staining. These bacterial isolates were affiliated to genus Bacillus (DS1 and DH1), Aeromonas (DP1 and DI4) and Zobellella sp. DD5, based on morpho-physiological characterization. Under optimized condition, Zobellella sp. DD5 produced 2.2 g l-1 polyhydroxyalkanoate from 3.0 g of dry cell biomass. The Fourier Transform Infra-Red Spectroscopy primary structural characterization study depict high intense peak at 1720.50 cm-1 corresponding to ester carbonyl (C=O) stretching vibration of polyhydroxybutyrate, which is the most common homopolymerof polyhydroxyalkanoates. Interpretation : This is the first report giving insight on non-growth associated polyhydroxybutyrate production (2.2 g l-1) by Zobellella sp. DD5 under submerged fermentation process, and further structural and thermal characterization is highly indispensibleforapplication ofpolyhydroxybutyrate.