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Simultaneous detection of infectious human echoviruses and adenoviruses by an In Situ nuclease-resistant molecular beacon-based assay

Applied and Environmental Microbiology (2012) 78(5) 1584-1588
DOI: 10.1128/AEM.05937-11
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Abstract

A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible within 3 h postinfection. © 2012, American Society for Microbiology.

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Dunams, D., Sarkar, P., Chen, W., & Yates, M. V. (2012). Simultaneous detection of infectious human echoviruses and adenoviruses by an In Situ nuclease-resistant molecular beacon-based assay. Applied and Environmental Microbiology, 78(5), 1584–1588. https://doi.org/10.1128/AEM.05937-11

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