Two-plasmid vector system for independently controlled expression of green and red fluorescent fusion proteins in Staphylococcus aureus

Abstract

We have constructed a system for the regulated coexpression of green fluorescent protein (GFP) and red fluorescent protein (RFP) fusions in Staphylococcus aureus. It was validated by simultaneous localization of cell division proteins FtsZ and Noc and used to detect filament formation by an actin-like ParM plasmid partitioning protein in its native coccoid host. © 2013, American Society for Microbiology.