Purification and characterization of a novel NADP-dependent branched- chain alcohol dehydrogenase from Saccharomyces cerevisiae

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Abstract

An NADP-dependent branched-chain alcohol dehydrogenase was purified from Saccharomyces cerevisiae var. uvarum grown under anaerobic conditions. Its quaternary structure is monomeric, and it has a molecular mass of 37 kDa and a pI of 5.9. A possible role of the enzyme in flavor production during alcoholic fermentation is discussed.

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Van Iersel, M. F. M., Eppink, M. H. M., Van Berkel, W. J. H., Rombouts, F. M., & Abee, T. (1997). Purification and characterization of a novel NADP-dependent branched- chain alcohol dehydrogenase from Saccharomyces cerevisiae. Applied and Environmental Microbiology. American Society for Microbiology. https://doi.org/10.1128/aem.63.10.4079-4082.1997

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