The Linker Region Plays a Regulatory Role in Assembly and Activity of the Vps4 AAA ATPase

Abstract

Background: Vps4 functions in eukaryotic cells in membrane fission reactions. Results: Mutational analysis suggests that the long linker region of Vps4 is not essential but regulates this ATPase. Conclusion: The binding to the substrate ESCRT-III drives formation of the active Vps4 complex. Significance: Understanding the mechanism of Vps4 function gives insights into protein trafficking, cytokinesis, and virus formation. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.