Inter-laboratory comparison of Cry1Ab toxin quantification in MON 810 maize by enzyme-immunoassay

Abstract

A laboratory ring trial was performed in four laboratories for determination of Cry1Ab toxin in leaf material of MON 810 maize using a standardised enzyme-linked immunoassay protocol. Statistical analysis was carried out by the ISO 5725-2 guidelines, sample standard deviation and standard error, within-laboratory and inter-laboratory SD and SE were calculated. Measured inter-laboratory average values were 12.5±4.0, 15.3±4.6 and 72.6±17.8 μg/g for three lyophilised samples, and 27.8±4.3 μg/g for a frozen sample, yet, Cry1Ab concentrations ranged 66.5-160.1% of the corresponding IA. Determined concentrations by in-house protocols were statistically not different in one laboratory and different in two laboratories from the corresponding values by the joint protocol. Results emphasise the importance of a standardised protocol among laboratories for comparable quantitative Cry1Ab toxin determination. However, even when using a standardised protocol, significant differences still occur among toxin concentrations detected in different laboratories, although with a smaller range of variation. © 2012 Copyright Taylor and Francis Group, LLC.