Comparison of the Immunomodulatory Properties of Root and Leaves of Arctium Lappa (Burdock) in Vitro

Abstract

Objective: The roots and leaves of Arctium lappa (burdock) have been used for different therapeutic purposes, especially for diseases linked to chronic inflammation. The present study was designed to evaluate and compare the immunomodulatory activities of root extract of burdock (REB) and leaves extract of burdock (LEB) in vitro. Material(s) and Method(s): For this purpose, PHA- or LPS-stimulated splenocytes were treated with different concentrations of REB or LEB and proliferation of splenocytes measured by MTT assay. The levels of IFN-gamma and IL4 in the supernatants of PHA-stimulated splenocytes determined using ELISA. We also studied the effects of REB and LEB on Nitric Oxide (NO) production by LPSstimulated macrophages using the Griess reagent. Result(s): Our findings showed that both REB and LEB have suppressive effects on LPS-stimulated splenocytes proliferation, IL-4 secretion from PHA-stimulated splenocytes, and NO production from LPS-stimulated macrophage and stimulatory effects on PHA-stimulated splenocytes proliferation, and IFN-gamma secretion from PHA-stimulated splenocytes. Although both REB and LEB had similar immunomodulatory affects in vitro, stronger immunomodulatory effects seen in REB. Conclusion(s): According to our results, we suggest that root of burdock is better option than leaves of burdock in modulation immune responses and inflammations.