Antioxidative enzymes during germination of two lines of Serbian spruce [Picea omorika (Panc.) Purkyně]

Abstract

Two lines of Picea omorika (Panc.) Purkyn? were compared with respect to germination percentage as well as specific activity and isoenzyme pattern of catalase, superoxide dismutase, and peroxidase (POD) during germination. Line A had a higher germination percentage and higher enzyme activities in dry seeds and seedlings compared to line C. Peroxidase activity was not detected in dry seeds, but measured up to 10 U/g and 28 U/g on the 7th day of germination in lines C and A, respectively. The most abundant POD basic isoform in seedlings of both lines (pI 8.2) was not found previously in needles of adult Serbian spruce trees of the same lines. .Poredjeni su procenat klijanja, specificna aktivnost i izoenzimski profil katalaze, superoksid dismutaze i peroksidaze u dve linije omorike [Picea omorika (Panc.) Purkyn?] u toku klijanja. Linija A je imala veci procenat klijanja i vece enzimske aktivnosti u suvom semenu i klijancima, u poredjenju sa linijom C. Peroksidazna aktivnost nije nadjena u suvim semenima, a povecavala se do 10 U/g i 28 U/g sed- mog dana klijanja u linijama C i A, respektivno. Najzastupljenija bazna izoforma peroksidaze u klijancima obe linije (pI 8.2) nije nadjena ranije u cetinama odraslih jedinki istih linija. .