Isolation and characterization genes in lobi-lobi (Flacourtia inermis) related to sugar metabolism

Abstract

Lobi-lobi (Flacourtia inermis) is probably a native plant to the Moluccas (Maluku). In Indonesia, lobi-lobi fruit is used for traditional medicine because it has a phenolic compound. Sugar metabolism involved several genes that control the photosynthesis pathway. This research aims to isolate and characterize genes in which related to sugar metabolism in Lobi-lobi. The lobi-lobi contigs have 694 452 contigs produced by the velvet-assembled genome. The sugar-related genes were isolated from the assembled-genomes database against to SwissProt Database using the BLASTX program. The six-sugar related-genes were characterized involved 36 contigs of Sucrose-phosphate synthase (SPS), six contigs of Sucrose-phosphatase (SPP), 46 contigs of Sucrose synthase (SUS), 36 contigs of Alkaline/neutral invertase (INV), four contigs of Cytosolic invertase (CINV), and 17 contigs of Beta-fructofuranosidase (CWINV), respectively. Comprehensive research on sugar metabolism was used in this study. Then, the data also revealed the biological functions of these genes in flowering time control in Lobi-lobi.