MiR-30a inhibits glioma progression and stem cell-like properties by repression of Wnt5a

Abstract

miR-30a has been found to be dysregulated in diverse cancers and involved in the regulation of tumor progression. However, there is scarce research on the role of miR-30a in glioma. In the present study, we assessed the expression level of miR-30a in glioma tissues and cell lines. The microRNA microarray analysis revealed low expression of miR-30a in glioma tissues and cells vs. The control. Furthermore, we found that stable miR-30a inhibited cell proliferation, G1 phase arrest and stem cell-like formation in glioma. Moreover, to investigate the molecular mechanism of miR-30a on glioma cell phenotypes, we identified Wnt5a as a new direct target gene for miR-30a by bioinformatic assay, luciferase assay and western blot analysis. Further functional studies suggested that miR-30a suppressed metastasis, sphere formation and glioma growth by targeting Wnt5a signal pathway. Collectively, our findings suggested for the first time that miR-30a may function as a tumor suppressor in glioma by targeting Wnt5a.