Chemical profiling and discrimination of medicinal himalayan iris species using direct analysis in real time mass spectrometry combined with principal component analysis

Abstract

24 Chemical Profiling and Discrimination of Medicinal Himalayan Iris Species Using Direct Analysis in Real Time Mass Spectrometry Combined with Principal Component Analysis Pratibha Singh1,2, Vikas Bajpai1, Abha Sharma3, Bikarma Singh4, Brijesh Kumar1,2∗ 1Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Sitapur road, Lucknow, Uttar Pradesh, 226031, India 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110025, India 3National Institute of Pharmaceutical Education and Research, Raebareli, Lucknow, Uttar Pradesh, 226301, India 4Biodiversity and Applied Botany Division, CSIR-Indian Institute of Medicine, Jammu, 180001, India Graphical Abstract Metabolic profiling of I. crocea, I. ensata, I. germanica, I. hookeriana, I. kashmiriana and I. spuria, was carried out using direct analysis in real-time mass spectrometry (DART-MS) to generate the chemical fingerprints for the differentiation. Phytochemical analysis showed presence of twenty-two flavonoids and isoflavonoids in the intact leaf and root of these species. The DART-MS data have been subjected to principal component analysis (PCA) which showed clear differentiation among the species and plant parts. It clearly indicated that the DART-MS technique followed by PCA is a quick and reliable method for the direct profiling and discrimination of Iris species and their plant parts.