Erratum: A new primer construction technique that effectively increases amplification of rare mutant templates in samples (BMC Biotechnol (2019) 19 (62) DOI: 10.1186/s12896-019-0555-1)

Abstract

Following publication of the original article [1], the author informed us that the legend for Fig. 2 was incorrect. Fig. 2 legend has been corrected in the original article. The correct legend for Fig. 2 is given below: Mutant sequence enrichment using stuntmer PCR. The abilities of stuntmers to detect four different mutation scenarios (single nucleotide mutation, two different single nucleotide mutations, and a deletion mutation) were tested under three different sample conditions (only wild-type, 90:10 ratio of wild-type:mutant, and 99:1 ratio of wild-type:mutant). In all cases, the stuntmers were able to detect the mutations even when the mutant templates were present in only 1% of the tested samples. The wild-type template was completely inhibited in the T790 M point mutation (a) and exon 19 deletion mutation (b). The arrow indicates where the deletion mutation occurred.