Determination of optimum wavelength and derivative order in spectrophotometry for quantitation of hydroquinone in creams

Abstract

UV derivative spectrophotometry was used for quantitative determination of hydroquinone in creams. The aim of this work was to investigate optimum wavelength and order of derivative, and to validate the proposed spectrophotometry method. The results of standard curves were calculated and statistically analyzed through the least squares method in the interval from 10.0 to 26.0 μg/mL, in the first, second, third and fourth order derivatives. The quantitative determination was carried out by using the zero-crossing (Z-C) and zero-peak (Z-P) methods. The proposed method is simple, of low cost and provides reliable results in order to be used in quality control of creams containing hydroquinone as active substance.