Formulation and Evaluation of Gentamicin-Loaded Transferosomal Gel

Abstract

Gentamicin is a broad-spectrum antibiotic that is utilized in treating infections caused by strains of Streptococci, Staphylococci, Pseudomonas, and gram-negative bacteria. Gentamicin, being a BCS class III drug possess poor permeability. The aim of the current research was to formulate and optimize Gentamicin-loaded topical transferosomal gel to enhance its permeability. The components such as, surfactants, edge activators, phospholipids were screened individually with drug. The transferosomal gel formulations were prepared by thin film hydration method. The formulations had particle size ranging from (113.98±2.51 nm) to (309.09±1.10nm), in vitro drug release ranging from (75.98±0.55%) to (97.13±0.24%), Zeta potential(-34.56mV) to (-13.18mV), PDI ranging from (0.205) to (0.542). The Optimization of Transferosomal gel was done on the basis of different concentrations of Carbopol 934 and HPMC k15 and the optimized formulation is characterized for viscosity, spreadability, SEM analysis, pH, drug content and in vitro drug release. The 0.1 % Gentamicin transferosomal gel had Spreadability 0.301±0.25 g.cm/sec, pH value is 6.1. Viscosity of optimized Gentamicin transferosomal gel was found to be 68521cps, and percentage drug release for was 98.59 ± 0.46. Finally, stability studies were carried out for prepared transferosomal gel and it was stable at30℃ ± 2℃ / 65 % ± 5 % RH for 90days. Key words: Gentamicin Transferosomal gel, Transferosomes, Topical, Antibiotic drug, Thin Film Hydration Technique.