Temperature-responsive regulation of the polycyclic aromatic hydrocarbon-degrading mesophilic bacterium Novosphingobium pentaromativorans US6-1 with a temperature adaptation system

Abstract

Survivability and tolerance of polycyclic aromatic hydrocarbon (PAH)degrading bacteria in harsh environments, especially under varying temperatures, are a bottleneck for the effective application of in situ bioremediation. In this study, a temperature adaptation system (TAS) was constructed by combining a customized thermotolerant system with a customized cold-resistant system to realize the temperature-responsive regulation of the PAH-degrading mesophilic bacterium Novosphingobium pentaromativorans US6-1. The innovative dual-pronged TAS strategy enabled the chassis strain to effectively tackle conditions under varying temperatures, ensuring robust biological activities across a broadened temperature spectrum and exhibiting the potential to realize the high-efficiency PAH degradation of N. pentaromativorans US6-1 in in situ bioremediation. Furthermore, the temperature-responsive regulation achieved using the TAS circuit is likely promising for creating intelligent microbial cell factories and avoiding precise temperature maintenance, making it highly useful for industrial applications.