Enzyme-immunoassay for the measurement of antigens using peroxidase conjugates

Abstract

Human and rat IgG were labelled with peroxidase. Procedures were established using these conjugates and insoluble antibody immunoadsorbents for the quantitation of humoral IgG. The basic principles of these procedures are those already developed for quantitative radio-immunoassay, enzyme activity measurement substituting radioactivity counting. The procedures allowed the determination of 10-200 ngs of antigen. The values obtained in unknown samples using these enzyme-immunoassays were in good agreement with those obtained using the quantitative single radial immunodiffusion technique. © 1972.