Effects of different plant hormones on callus induction and plant regeneration of miniature roses (Rosa hybrida L.)

Abstract

Miniature roses (Rosa hybrida L.) are increasingly popular flowering potted plants. In this study, we used leaves of new cultivars of Rosa hybrida as explants and MS medium as basal medium, to explore optimal combinations and concentrations of growth regulators on callus, adventitious buds and root induction. Our results demonstrated that MS medium supplemented with 3.0mgL-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0mgL-1 6-Benzylaminopurine (6-BA) could result to 100% callus induction ratio. Furthermore, we showed that 6-BA was of essential importance during the induction of adventitious buds in R.hybrida. On MS medium containing 1.0mgL-1 6-BA, (0.05-0.5) mgL-1 naphthaleneacetic acid (NAA) and (0.02-0.2) mgL-1 Thidizuron (TDZ), adventitious buds could be regenerated from calli and the redifferentiation ratio reached 92.6%. Of note, our data supported the indispensable role of auxin during rooting induction, because 1/4MS medium enabled 100% rooting frequency when 0.1mgL-1 NAA was supplemented. In conclusion, we have established a tissue culture system, by which superior frequency of tissue regeneration and biologically similar cultures of R.hybrida could be achieved.