Sequence analysis of the cytochrome oxidase I gene in Aedes albopictus isolated from Taman Bukit Kinrara and PJS7, Selangor

Abstract

Aedes albopictus is one of the most invasive mosquitoes in the world that harbors and can transmit many arboviruses, most notably dengue and chikungunya virus. In recent time, Ae. aegypti has gained more attention during dengue outbreaks compared to its counter parts, obscuring the role of Ae. albopictus as a vector. Moreover, existing data regarding Ae. albopictus is also currently limited in Malaysia. Hence, the present study was conducted to determine the genetic diversity of Ae. albopictus using molecular techniques from two dengue infested areas in Subang Jaya, Selangor, namely Taman Bukit Kinrara (TBK) and PJS7. Cytochrome oxidase 1 (CO1) gene from field collected mosquitoes were analyzed and compared to the USM laboratory strain (F135) together with sequences from the GenBank. Results from this study revealed that the field collected mosquitoes from TBK and PJS7 are genetically similar with each other. However, the samples exhibited polymorphism with the laboratory strain by 47 variable nucleotide sites. Our local samples are related with the Ae. albopictus populations from India, probably due to its migration across these two regions via several human activities. This study shows that the CO1 gene is a valuable marker for the detection of Ae. albopictus and can be utilized to study its worldwide geographical distribution. Further analysis is strongly recommended using larger sample size and different localities to validate and substantiate our findings.