A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using 15N-labeled polyamines. © 2007 Pharmaceutical Society of Japan.
CITATION STYLE
Samejima, K., Otani, M., Murakami, Y., Oka, T., Kasai, M., Tsumoto, H., & Kohda, K. (2007). Electrospray ionization and time-of-flight mass spectrometric method for simultaneous determination of spermidine and spermine. Biological and Pharmaceutical Bulletin, 30(10), 1943–1946. https://doi.org/10.1248/bpb.30.1943
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