Evaluation of Antimicrobial, Cytotoxic and Larvicidal Activity of Zygophyllum Coccineum North Sinai, Egypt

Abstract

The antimicrobial larvicidal and cytotoxic properties of Zygophillum coccinium collected from desert of north Sinai, Egypt. Antimicrobial activity of Z. coccinium extracts showed that ethyl acetate extract was the most potent antimicrobial effect on bacterial and fungal test organisms against P. aeruginosa with inhibition zone 20 mm and F. moniliforme with inhibition zone 22 mm. Cytotoxic effect of Z. coccinium extracts indicated that acetone extract showed maximum cytotoxic activity on HeLa cell line, whereas methanol extract showed maximum cytotoxic activity on MCF-7 cell line with cell line viability 13.36 and 35.19%, whereas the results of IC50 in HeLa cell line showed that the boiled water ethyl acetate extract had the most potent IC50 with 6.25 µg/ml. the results of IC50 in MCF-7 cell line the petroleum ether extract had the most potent IC50 with 18.75 µg/ml. Ethyl acetate extract of Z. coccineum were the most potent medicinal plants as antibacterial effect with MIC 12.5 µg/ ml. Ethyl acetate with 200 ppm conc. of Z. coccineum is the lowest conc. possess toxic effect against 3rd instar larvae of Culex pipiens The results of column chromatography the antimicrobial activity of Z. coccineum against K. pneumonia showed that the highest fraction with antimicrobial activity was fraction No 3 with inhibition zone 22 mm. Spectroscopic characterization of antimicrobial agent. The chemical compound structure of of Z. coccineum was identified as was suggested as 2-(3,4-Dihydroxyphenyl)-3,5,7-trihydroxy-6-methoxy-4-benzopyrone.