Preparation and Characterization of Phosvitin from Hen's Egg Yolk Granule

Abstract

Soluble delipidated granule was fractionated into mainly two components by gel filtration and each component was purified by DEAE-cellulose chromatography. The phosvitins so prepared were designated as phosvitin 1 and phosvitin 2. Phosvitin 1 contained 59.2% serine and 10.2% phosphorus, while phosvitin 2 had 51.5% serine and 8.9% phosphorus. Phosvitin 1 was dissociated into nine protein fractions ranging in molecular weights from 1.33 x 104∼ 1.36 x 105 daltons, in the SDS-polyacrylamide gel electrophoretic pattern. On the other hand, Phosvitin 2 was dissociated into thirteen bands ranging in molecular weights from 1.1 x 104∼ 1.37 x 105 daltons, in the SDS-polyacrylamide gel electrophoretic pattern. On the contrary, the major component of phosvitin 1 has a molecular weight of 2.29 x 104 when determined by gel filtration with 6 m guanidine hydrochloride, while the major components of phosvitin 2 have molecular weights of 2.29 x 104 and 1.0 x 104. From these results, it seems that phosvitin is composed of several subcomponents. © 1984, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.