Direct quantitative measurement of the C==O···H-C bond by atomic force microscopy

Abstract

The hydrogen atom-the smallest and most abundant atom-is of utmost importance in physics and chemistry. Although many analysis methods have been applied to its study, direct observation of hydrogen atoms in a single molecule remains largely unexplored. We use atomic force microscopy (AFM) to resolve the outermost hydrogen atoms of propellane molecules via very weak C==OH-C hydrogen bonding just before the onset of Pauli repulsion. The directmeasurement of the interaction with a hydrogen atom paves the way for the identification of three-dimensional molecules such as DNAs and polymers, building the capabilities of AFMtoward quantitative probing of local chemical reactivity.