Evaluation of soil origin Pseudomonas sp. For production of bioactive compounds

Abstract

Nowadays, bioactive compounds produced by bacteria consider a source of new remedy to eliminate antibiotic-resistant bacteria. Therefore, the main purpose of this study was isolation and identification of soil origin Gram-negative bacteria and their evaluation for production of bioactive compounds. In general, three bioactive compounds producing bacteria were isolated from hundred and twenty five soil samples. These strains identified as Pseudomonas aeruginosa, Pseudomonas putida and Comamonas testosteroni based on phenotypic and 16 SrRNA Gene sequencing methods. The bioactive compounds produced by isolated strains were partially purified and their activities evaluated at different pHs and temperatures. Then, their antimicrobial spectrums assessed against different pathogenic microorganisms. The results obtained indicated that the best temperature and pH for activity of the bioactive compounds were 40-50°C and 6.5-7.5, respectively. In addition, bioactive compounds produced by P. aeruginosa and P. putida showed an antimicrobial property against Staphylococcus aureus, Bacillus cereus, Corynebacterium sp. Listeria monocytogens, Klebsiella pneumonia, Shigella dysentriae and Proteus mirabilis; however, E. coli was resistant to them. Although all the bacteria were resistant to the bioactive compound produced by C. testosteroni, Aspergillus niger and Alternaria sp. were sensitive. Overall bioactive compounds produced by Pseudomonas sp. showed activity against some pathogenic bacteria as well as fungi and might be considered an alternative therapeutics not only in pharmaceutical but also as a preservative in food industries. © 2013 Asian Network for Scientific Information.