Experimental study upon the virulence of infectious microbial agents involved in violent deaths presenting septic states

Abstract

The study of bacterial pathogeny represents a very important issue for the forensic specialists, clinicians, managers and healthcare policymakers. The purpose of this experimental research is to study the virulence of some infectious microbial agents (Pseudomonas Aeruginosa, Staphylococcus Aureus, Escherichia Coli, Proteus Mirabilis, Erysipelothrix Rhusiopathiae), some of them frequently involved in violent deaths presenting septic states, by inoculating them in white mice Mus Musculus (MMs), in the presence of competing factors of death, reproducing this way a model of human violent death consecutive of septic complications that occur in the evolution of some traumas.The results of the study show that the survival period of the MMs depends on the microbial agent of the inoculated strain, the dilution of the culture suspension, the presence of associated lesions, age. In decreasing order, according to the value of LD50, the most virulent strains are Erysipelothrix Rhusiopathiae, Escherichia Coli, Proteus Mirabilis, followed by Staphylococcus Aureus and Pseudomonas Aeruginosa. The lethality index increases proportionally with the virulence of the etiologic agent and the presence of the associated lesions, confirming once again the similarity with physiopathological mechanisms that exist in human pathology.