Regulation of vasomotion of arterioles and capillaries in the cat spinal cord: Role of α actin and endothelin-1

Abstract

Ring-shaped vasoconstrictions of arterioles at their branching sites have often been reported in vascular corrosion casts of the brain and spinal cord in rats and cats. It is surmised that smooth muscle cells in arteriolar walls could regulate the blood flow by changing the diameter of the lumen (ie vasomotion). However, few reports have described vasomotion at the capillary (capillaries have no smooth muscle cells). Also, there have been no reports on endothelin-1 in the arterioles and capillaries of the spinal cord. This study was designed to determine (1) the electron microscopic architecture of vasomotion; (2) the immunohistochemical identification of α actin and endothelin-1 in the arterioles and capillaries of the spinal cord. Twenty-seven adult mongrel cats were used to study vascular corrosion casts at the lumbosacral spinal cord segments immunohistologically and through scanning electron microscopic observations. Sections of the spinal cord were stained with monoclonal anti-α actin and endothelin-1 antibodies. Vascular corrosion casts demonstrated two types of vasomotion: a sausage-like peristalsis and a ring-shaped vasoconstriction at the arteriole and capillary levels. In the immunohistological study, α actin and endothelin-1 were identifiable in the vascular wall at the bifurcation, and pericytes were found to contain microfilaments of α actin. The ring-shaped vasoconstriction might be regulated by smooth muscle cells in arterioles and by pericytes in capillaries by releasing endothelin-1.