Electrochemical monitoring of the interaction between mitomycin C and DNA at chitosan-carbon nanotube composite modified electrodes

Abstract

Single-walled carbon nanotube (CNT) and chitosan composite (chitosan∗CNT) based sensors were developed as DNA biosensors, and then they were applied for electrochemical investigation of the interaction between the anticancer drug mitomycin C (MC) and DNA. The oxidation signals of MC and guanine were monitored before and after the interaction process by differential pulse voltammetry (DPV). The DPV results were in good agreement with those of electrochemical impedance spectroscopy (EIS). Analytical parameters such as DNA concentration, MC concentration, and MC interaction time with DNA were optimized. The detection limits were 6.85 μg/mL for DNA and 11.01 μg/mL for MC.