Electrochemiluminescence immunoassay for progesterone by using a heterologous system in plasma bovine

Abstract

Aim: The present study describes the use of electrochemiluminescence (ECL) immunoassay method with specific kit human progesterone for measuring plasma progesterone in cattle. Materials and Methods: Nine Holstein-Friesian females were selected and artificially inseminated (AI). Blood samples were collected from the coccygeal vessels into tubes containing EDTA at day 0 (n = 5, the control group) and day 90 (n = 4, diagnosed pregnant by rectal palpation) after AI. The day of AI was considered as day 0 for the calculation of pregnancy day. The samples were immediately centrifuged (15 min at 1500 × g), and the plasma was stored at -20°C until assay. The assay of progesterone was carried out by a method of competition immunological type in heterogeneous phase. The antibodies of capture and revelation are monoclonal specific to progesterone of human origin. Results: The progesterone (P4) concentration in the whole female was physiological. The results of inter- and intraassay coefficients of variation were 16.6% and 6.7%, respectively. The values of accuracy and parallelism obtained were satisfactory. Conclusion: The preliminary results show clearly that human progesterone ECL kit can be used to measure P4 in plasma bovine.