Comparative Mapping and 3'UTR SNP detection of ANGPTL4 gene in beef cattle

Abstract

Angiopoietin-like 4 (ANGPTL4), a member of the angiopoietin-like gene family, encodes a glycosylated, secreted protein with a fibrinogen C-termmal domain which is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. ANGPTL4 is a serum hormone directly involved in regulating angiogenesis, lipid metabolism, glucose metabolism, cancer and metabolic diseases. In this study, Radiation Hybrid (RH) cloning board technique, PCR-SSCP and DNA sequencing methods were employed to map bovine ANGPTL4 gene and examine the single nucleotide polymorphisms (SNPs) at the 3'UTR of this gene in 281 cattle from seven breeds. Here, the bovine ANGPTL4 gene was located between the framework marker CA006 and DIK4204 on BTA7 with a map distance of 2.3 3 cR to the former and an interval of 8.54 cR to the latter. A novel mutation (NC007305.3: g.C6640T) was revealed which constructed three genotypes (CC, CT and TT). This locus proved to be significantly associated with Average Daily Gam (ADG), Rib-eye Area (REA), Intramuscular Fat (IMF) (p<0.05) and Beef Performance Index (BPI) (p<0.01) in the analyzed populations. Meanwhile, individuals with genotype TT were significantly higher than those with CC in BPI, ADG and REA while it was reversed for genotype TT and CC in the IMF which proved that bovine ANGPTL4 gene had a positive effect on production traits. Hence, genotype TT and CC could be regarded as molecular markers for BPI, ADG and REA and IMF, respectively. © 2011 Medwell Journals.