Improvement of L-arabinose fermentation by modifying the metabolic pathway and transport in Saccharomyces cerevisiae

Abstract

The L-arabinose utilization pathway was established in Saccharomyces cerevisiae, by expressing the codon-optimized araA, araB, and araD genes of Lactobacillus plantarum. After overexpressing the TAL1, TKL1, RPE1, RKI1, and GAL2 genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0.075 h-1, a maximum specific L-arabinose consumption rate of 0.61 g h-1 g-1 dry cell weight, and a promising ethanol yield of 0.43 g g-1 from L-arabinose fermentation. © 2013 Chengqiang Wang et al.