Isolation and identifi cation of pharmaceutically active lipase producing Bacillus spp. from mangrove sediments against Methicillin Resistant Staphylococcus aureus isolated from wound of patients

Abstract

Ninety two clinical wound samples were collected, among, 50 positive Staphylococcus aureus were isolated. These isolates were characterized morphologically and biochemically. Twenty three antibiotics were used to determine susceptibility patterns by disc diffusion method. The multiple antibiotic resistances (MAR) index were calculated according to the MAR index formula ranged from 0.21 to 0.78. All the 50 isolates of MRSA were showed MAR index in between these ranges. Among these MAR index the pathogenic isolates (100%) were resistant of Penicillin, while trimethoprim showed resistance (86%), cephoxitin (80%), kanamycin (78%), vancomycin and cepfodoxamine (72%), moxalactam and quinupristin (70%), cotrimoxazole (66%), methicillin (64%), novobiocin (62%) and erythromycin (56%) and there was no resistance found to chloramphenicol, and rifampicin. More than 65% resistance MRSA isolates were selected for plasmid isolation. Natural products are boundless source for important novel compounds having antagonistic activity against pathogenic organisms. Marine environment covers almost 70% of the earth surfaces. Organisms present in these environments are extremely rich sources of bioactive compounds. The ocean remains as an unexploited source of many drugs and pharmacologically active substances. Microbial enzymes have many advantages over the animal and plant enzyme, firstly; they are economical and can be produced on large scale within the limited space and time. Secondly, they are capable of producing a wide variety of enzymes; they can grow a wide range of environmental condition. Enzymes have many roles in the pharmaceutical and diagnostic industries. The bacteria Bacillus spp. was isolated from the mangrove sediment, for the lipase production. These enzymes may inhibit the growth of MRSA. Recently with the advent of biotechnology, there has been a growing interest and demand for enzymes with the novel properties.