Ten species specific microsatellite loci for Lahontan cutthroat trout, Oncorhynchus clarki henshawi

Abstract

Ten polymorphic microsatellite loci (containing tri and tetra-nucleotide repeats) were developed for the Lahontan cutthroat trout (Oncorhynchus clarki henshawi), a subspecies of cutthroat trout listed as threatened under the United States Endangered Species Act. Polymorphism was assessed for 445 individuals from 12 populations representing eight watersheds spread throughout the three Distinct Population Segments defined for this subspecies. All loci were polymorphic (X̄ = 19, range 7-26 alleles). All loci were in Hardy-Weinberg equilibrium (HWE) except for one locus (OCH 9) in a single population (P < 0.00014 after Bonferroni correction for multiple tests).